Screening Lipolytic from Soil Bacterial Contaminated Oil

Lipase (triasilglycerol acylhydrolase, E.C, 3.1.1.3) is an enzyme can hydrolyze long chains of triglycerides[1]. a water-soluble and naturally lipase catalyze the hydrolysis ester bonds in water insoluble lipid substrate long-chain acylglycerol synthesis, diacylglycerol, monoacylglycerol, glycerol[2]. Hydrolase be used potentialy produced fatty acid which precursors chemical industry[3]. The research aims to study lipolytic activity bacteria isolated from soil contaminated with oil. Screening was performed Rhodamin-B agar plate containing olive determined by spectrofotometry method toward p-nitrofenil palmitat as substrate. result this showed that there are 15 150 isolates oil confirmed observing zone formed around growth colony on Rhodamin B One identified higher 7,41 U/ml